KOD Xtreme Hot Start DNA Polymerase successfully amplifies challenging DNA templates with up to 90% GC content. Each kit provides 200 U KOD Xtreme Hot Start DNA Polymerase, an optimized buffer and dNTPs sufficient for 200 amplification reactions.

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μl) consisted of 2× KOD Xtreme™ PCR buffer, 2 mM dNTPs, 10 pmol/μl each forward and reverse primer, 1.0 U/μl KOD Xtreme™ Hot Start DNA polymerase, Genomic DNA was isolated from the fungal mycelia using the GF-1 nucleic acid U/µl KOD Xtreme™ Hot Start DNA polymerase, and 100 ng/µl template DNA, The KOD Xtreme™ Hot Start DNA Polymerase* kit is an optimized PCR enzyme for the amplification of long or GC-rich DNA templates, including mouse tail-tips, Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and The reaction conditions are compatible with direct addition of primers and KOD Hot Start DNA Polymerase or KOD XL. DNA Polymerase for amplification of the 15 Oct 2013 “Typically DNA polymerases are stratified into high-fidelity and those for routine DNA polymerase from Thermococcus species KOD [kodakaraensis] with Agilent's PfuTurbo Cx Hotstart enzyme “can read through uraci The PCR error ratio of KOD -Plus- is approximately 80 times less than that of Taq DNA polymerase.

KOD -Plus- is based on DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1(1)(2) KOD -Plus- exhibits excellent high PCR fidelity and efficiency. The enzyme solution of KOD -Plus- contains two types of anti-KOD DNA polymerase antibodies that inhibit polymerase and 3'→5' exonuclease activity, thus allowing for Hot Start PCR(3).

| Match Criteria: Product Name. 71975-M. Ultra high fidelity dnapolymerase designed for the most challenging PCR applications including: crude sample PCR, amplification of long strand and highly GC-rich DNA templates.

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KOD Xtreme polymerase enables convenient ambient-temperature This protocol uses MilliporeSigma's KOD Xtreme™ Hot Start DNA Polymerase, which allows for high fidelity PCR using a simple three-step thermocycle. It is important to complete the entirety of this protocol in one go, rather than delay performing the PCR, digestion, or transformation.

Effective for the amplification of GC-rich targets. Hotstart 17 May 2018 PCR amplification of DNA templates from plasmids using KOD Xtreme™ Hot Start DNA Polymerase.
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High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications. Sigma-Aldrich. KOD Xtreme Hot Start DNA Polymerase 71975-M Ultra high fidelity dnapolymerase designed for the most challenging PCR applications including: crude sample PCR, Sigma-Aldrich pricing 71842.
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Merck™ Novagen™ KOD DNA Polymerase KOD DNA Polymerase DNA Polymerases PCR Reagents and Kits. Shop online for a wide selection of EMD Millipore Novagen KOD Hot Start DNA Polymerase For PCR amplification of long strand and GC-rich DNA templates.

It is important to complete the entirety of this protocol in one go, rather than delay performing the PCR, digestion, or transformation. Article Title: Improved allele-specific PCR assays for detection of clarithromycin and fluoroquinolone resistant of Helicobacter pylori in gastric biopsies: identification of N87I mutation in GyrA Article Snippet: The mix for KOD Xtreme™ Hot Start DNA Polymerase was performed using 2.4 μL of distilled water, 10 μL of 2X reaction Xtreme buffer, 4 μL of dNTPs (200 μmol/L each), 0.3 μmol/L KOD Hot Start DNA Polymerase, KOD XL DNA Polymerase, KOD Xtreme™ Hot Start DNA Polymerase, NovaTaq™ DNA Polymerase, NovaTaq™ Hot Start DNA Polymerase, One Step RT-PCR Kit Use of these products is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224; KOD Xtreme Hot Start DNA Polymerase Other non-specified industry: Analytical reagent. Manufactured by EMD Chemicals, Inc 441 Charmany Drive Madison, WI 53719 (608) 238-6110 (800) 207-0144 FAX: (608) 238-1388 P.0. Box 12087 La Jolla, CA 92039-2087 (858) 450-5558 (800) 854-3417 KOD -Plus- is based on DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1(1)(2) KOD -Plus- exhibits excellent high PCR fidelity and efficiency. The enzyme solution of KOD -Plus- contains two types of anti-KOD DNA polymerase antibodies that inhibit polymerase and 3'→5' exonuclease activity, thus allowing for Hot Start PCR(3). KOD Hot Start DNA Polymerase, KOD XL DNA Polymerase, KOD Xtreme™ Hot Start DNA Polymerase, NovaTaq™ DNA Polymerase, NovaTaq™ Hot Start DNA Polymerase, One Step RT-PCR Kit Use of these products is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224; Kod Hot Start Dna Polymerase found in: KOD Hot Start DNA Polymerase, KOD Xtreme Hot Start DNA Polymerase, KOD Hot Start Master Mix, KOD Hot Start DNA.. Hot start PCR is a modified form of conventional polymerase chain reaction (PCR) that reduces the presence of undesired products and primer dimers due to non-specific DNA amplification at room (or colder) temperatures.

KOD Xtreme™ Hot Start DNA Polymerase SDS Safety Data Sheet for KOD Xtreme™ Hot Start DNA Polymerase 71975. Material Safety Data Sheet or SDS for KOD Xtreme™ Hot Start DNA Polymerase 71975 from MilliporeSigma for download or viewing in the browser.

KOD Xtreme™ Hot Start DNA Polymerase Ultra high fidelity dnapolymerase designed for the most challenging PCR applications including: crude sample PCR, amplification of long strand and highly GC-rich DNA templates. - Find MSDS or SDS, a COA, data sheets and more information. Se hela listan på sigmaaldrich.com KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates. KOD Xtreme Hot Start DNA Polymerase. The KOD Xtreme™ Hot Start DNA Polymerase* kit is an optimized PCR system for the amplification of long or GC-rich DNA templates. The unique formulation enables you to amplify directly from minimally processed samples; allowing highly efficient crude sample PCR target amplification.

High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications. KOD Hot Start DNA Polymerase MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. ready-to-use solution, Ready-to-use 2X mixture, containing KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4, optimized for convenient high fidelity PCR., suitable for PCR. Sigma-Aldrich.